The campaign will set off from the UN climate change conference (COP26) in Glasgow and tour the country in the build up to the day itself on 4 December.
Among the features this year is a fleet of eco-vehicles that reflect the sustainable switches many small businesses have made or are considering.
This comes as 97% of small businesses say sustainability is important to their business, according to research by campaign supporters BT Skills for Tomorrow and Small Business Britain.
The tour will deliver a hybrid format of online and in-person activity. This will include virtual workshops, webinars and mentoring on a range of small business topics, as well as visits and live-streamed interviews with local businesses in cities including Manchester, Belfast, Cardiff, and Edinburgh, as well as Whitley Bay and Hereford.
Michelle Ovens, director of Small Business Saturday, said: “We are so excited to get back on the road with this new tour and put the spotlight on fantastic small businesses and their communities, as well as opening up skills and networking opportunities to millions of entrepreneurs using a new hybrid format.”